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After a washing step, selected antibodies recognizing antigen are eluted and then transformed in bacteria for amplification. Another cycle of phage display antibody selection can start. 3 to 4 rounds of selection are necessary to obtain specific antibodies.
Hybrigenics' Phage Display Antibody selection features a unique process resulting in the enrichment of antigen-specific clones. Importantly, native full–length antigen is not adsorbed in plate and therefore preserved during multiple rounds of selection. Several antibodies recognizing different epitopes of an antigen can be identified. As a result, our antibodies are more likely to recognize endogenous antigens for in vitro and in vivo applications, such as immunofluorescence, live cell imaging, video microscopy or proteasome addressing. This also presents the great advantage of selecting putative conformational antibodies.
Our basic Hybribody offer includes the validation of the selected antibodies by ELISA. Like our Phage Display antibody selection, our ELISA assay uses a native antigen that is not adsorbed. We offer you a range of additional services to test your validated antibodies in an immunofluorescence assay or as intrabodies for live cell imaging, videomicroscopy and many more applications. By fusion of a Fc fragment from the species you need, we can also construct minibodies for you. Contact our team for more details. Another Phage Display Antibody selection process dedicated to cell surface receptors like GPCRs is also available.