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Don’t be disappointed by inefficient antibodies, get IF-validated ones.
To identify the ELISA-validated Hybribody selected from the hs2dAB library that will also work in IF, we express your antigen in cells as a fusion with a fluorescent protein targeted at a discrete subcellular localization. The Hybribody to test is tagged, expressed as an E.coli supernatant and its colocalization with the antigen is detected with a secondary labeled anti-tag antibody by fluorescence microscopy.