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Are you looking for a recombinant antibody you can express in mammalian cells? Hybrigenics selects validated intracellular nanobodies for you: intrabodies
To identify functional intracellular nanobodies, Hybrigenics runs a selection from its synthetic VHH library (hs2dAb) using Phage Display coupled with Yeast Two-Hybrid. Find more technical information on Antibody Phage Display Selection and Intrabody Enrichment pages. This combined screening favors the enrichment in intrabodies that will be functional in cell because we select nanobodies that are expressed and properly folded in the eukaryotic model organism S. cerevisiae.
To validate the intracellular expression of nanobodies and the recognition of endogenous antigens, we express both your antigen and pre-selected VHH nanobodies in cells as fusions with fluorescent proteins and detect their colocalization by fluorescence microscopy.
For more information, please contact us.
For intrabody selection
For intrabody validation only