- Our services
- Synthetic VHH Library
- Our technologies
- Our references
Are you looking for a recombinant antibody you can express in mammalian cells? Hybrigenics selects validated intracellular antibodies for you.
To find functional intracellular antibodies, Hybrigenics makes a selection from a synthetic VHH library (hs2dAb) by Phage Display coupled with Yeast Two-Hybrid. Find more technical information on Antibody Phage Display Selection and Intrabody Enrichment pages. This double screening favors the enrichment in intrabodies that will be functional in cell because we select antibodies that are expressed in the nucleus of the eukaryotic model organism S.cerevisiae.
To validate the intracellular expression of antibodies and the recognition of endogenous antigen, we express both your antigen and pre-selected VHHs in cells as fusions with fluorescent proteins and detect their colocalization by fluorescence microscopy.
For more explanations, do not hesitate to contact our teams.
For intrabody selection
For intrabody validation only