The future of
single-domain antibodies



Minibody Cloning & Production

Process antibody cloning & production

Now that you have your custom VHH, obtain the corresponding human, mouse, rabbit or goat “Minibodies”!

Key benefits

  • Multispecies antibody in a single clone
  • Versatile & flexible use for a vast array of experiments (PLA, ELISA…)
  • Enhanced avidity

How does it work?

As we provide you with VHHs selected from a synthetic library, we are able to fuse them with Fc fragments from human, mouse, rabbit or goat to obtain a so-called “Minibody". Minibodies from different species have the same binding regions and retain the same affinity for the Ag.

Let us know about the minibody you need.

Minibody Cloning & Production

Material required

Deliverables

  • Non-purified minibody supernatants from HEK cells (5 ml usable at 1/100 to 1/500 for IF)
  • cDNA clones of the minibodies for further production

Reference

A multi-Fc-species system for recombinant antibody production. Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dübel S, Perez F. BMC Biotechnoly. 2009 Feb 26; 9:14. doi: 10.1186/1472-6750-9-14.

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