- Our services
- Synthetic VHH Library
- Our technologies
- Our references
Do you need an affine antibody recognizing your cell surface receptor? It is not a problem for us to select it.
Hybrigenics can select antibodies by Phage Display directly on living cells when in vitro selection is not possible e.g for receptors with transmembrane domains like GPCR's. The hs2dAb library coding 3.109 VHHs is expressed at the phage surface and incubated with the cell expressing the antigen of interest. After a washing step with cells that do not express the antigen, the elution of the specific antibodies is performed and their enrichment is measured. 3 to 4 rounds of selection are performed.
Selected antibodies are validated by immunofluorescence (IF). Each antibody is tagged and expressed as an E.coli supernatant. Its colocalization with the antigen is detected with a secondary labeled anti-tag antibody by fluorescence microscopy.
Selected VHHs can also be validated by Phage FACS. After Phage Display selection, cells are incubated with an anti-M13 antibody labelled with a fluorescent marker and sorted in a FACS. Fluorescence signal is compared between cells expressing the antigen of interest and cells which are not.